| Congresso Brasileiro de Microbiologia 2023 | Resumo: 320-1 | ||||
Resumo:The decline in the jaguar population confirms how much the species is vulnerable to extinction in Brazil, also indicating the degradation of the environmental integrity and quality of its natural habitat. Studies claim that large felids are susceptible to Feline Panleukopenia Virus (FPV) and beind presumptively diagnosed clinically in Brazil. A free-living jaguar (Panthera onca) cub was found unconscious and rescued due to a possible hit-and-run in the savannah of Mato Grosso. Approximately two months after the rescue, the animal was walking normally, with no neurological or laboratory abnormalities, and was thus transferred to a larger enclosure, for its well-being. Three weeks after transfer to the enclosure, the animal presented it exhibited clinical and hematological signs consistent with FPV infection. Whole blood and rectal swab samples were collected for the molecular diagnosis of FPV. The following day, the animal died and was referred for necropsy, which revealed changes suggestive of parvovirus infection, like enteritis, multifocal crypt necrosis, moderate infiltration of mononuclear cells, shortening of intestinal villi and lymphoid depletion. DNA extraction from the rectal swab was performed using the MagMAX Sample Extraction Kit (Thermo Fisher®), according to the manufacturer's instructions. PCR was performed which amplifies a 1127bp fragment (a gene that encodes a fragment of the VP2 viral capsid protein). The product obtained by PCR was purified using the GE Healthcare IllustraTM GFXTM Kit and used in the sequencing reaction, together with BigDye terminator ready reaction cycle sequencing (Applied Biosystems) in the automatic sequencer ABI 3500 Genetic Analyzer (Applied Biosystems). The sequence was matched and deposited in the GenBank database using BLAST on the NCBI server (http: www.ncbi.nlm.nih.gov/BLAST), showing 99.61% similarity between the FPV and CPV-2 sequences (GenBank MZ883094). Phylogenetic analysis was performed by comparing amino acid and nucleotide sequences with other parvoviruses described in GenBank, subsequently aligned using software (Muscle, Gbloks, and T-Coffe), and phylogenetically analyzed using PhyML and TreeDyn programs. Due to habitat restrictions, certain diseases threaten wild cats and habitat encroachment by domestic animals can alter the pattern of spread of pathogens. We highlight the importance of the molecular diagnosis and phylogenetic analysis of FPV to elucidate how it has reached wild felids. Palavras-chave: jaguar, molecular diagnosis, Panleukopenia, phylogenetic analysis Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||